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Glutathione and glutathione disulfide – their biomedical and
Jul 28, 2023 · This review article covers the basic chemistry, physiological functions, and disease implications of glutathione (GSH) and its oxidized form glutathione disulfide (GSSG). It also …
Link.springer.comGlutathione Metabolism and Its Implications for Health
GSH displays remarkable metabolic and regulatory versatility. GSH/GSSG is the most important redox couple and plays crucial roles in antioxidant defense, nutrient metabolism, and the …
Jn.nutrition.orgGlutathione and glutathione-dependent enzymes: From …
Dec 1, 2023 · The GSH/GSSG ratio (or, in other words, the GSSG/2GSH couple) determines the cell redox state; notably, control of cell redox homeostasis and balance represents an …
Sciencedirect.comGlutathione-Related Enzymes and Proteins: A Review - PMC
Feb 2, 2023 · Basic reaction mechanisms of glutathione peroxidase (GPx), and glutaredoxin in (de)glutathionylation using (GSH)GSSG, respectively, and reduction of GSSG by the activity of …
Ncbi.nlm.nih.govGlutathione: Overview of its protective roles, measurement, and
Most of the GSH in antioxidant defense in cells is utilized by three members of glutathione peroxidase (GPx) family ( Brigelius-Flohe, 1999) and by one of the peroxiredoxins (Prdx 6). …
Ncbi.nlm.nih.govAssessment of glutathione/glutathione disulphide ratio and S
Nov 1, 2017 · The GSH/GSSG ratio provides a reliable estimation of cellular redox status. • GSH also plays a prominent role in protein redox regulation via S-glutathionylation. • We discuss …
Sciencedirect.comUnraveling the Potential Role of Glutathione in Multiple Forms of …
Jun 10, 2019 · Glutathione peroxidase (GPX) converts H 2 O 2 and Lipid-OOH to H 2 O and Lipid-OH where GSH is oxidized to GSSG, and glutathione reductase (GR) reduced GSSG to …
Ncbi.nlm.nih.govGlutathione - Wikipedia
In healthy cells and tissue, more than 90% of the total glutathione pool is in the reduced form (GSH), with the remainder in the disulfide form (GSSG). 80-85% of cellular GSH is in the cytosol and 10-15% is in the mitochondria.
En.wikipedia.orgReal-time monitoring of glutathione in living cells using genetically
Jan 22, 2020 · Reduced glutathione (GSH) level inside the cell is a critical determinant for cell viability. The level of GSH varies across the cells, tissues and environmental conditions.
Nature.comThe glutathione system and the related thiol network in - PubMed
The ratio of the reduced to disulfide glutathione redox couple (GSH:GSSG) is a key indicator of the redox environment and its associated cellular health. The reaction mechanisms of …
Pubmed.ncbi.nlm.nih.govMeasurement of oxidized/reduced glutathione ratio - PubMed
The ratio of reduced GSH to oxidized GSH (GSSG) is an indicator of cellular health, with reduced GSH constituting up to 98% of cellular GSH under normal conditions. However, the …
Pubmed.ncbi.nlm.nih.govThe fairytale of the GSSG/GSH redox potential - PubMed
Background: The term GSSG/GSH redox potential is frequently used to explain redox regulation and other biological processes. Scope of review: The relevance of the GSSG/GSH redox …
Pubmed.ncbi.nlm.nih.govGlutathione as an Antioxidant and Regulatory Molecule in Plants …
Jan 10, 2009 · The high ratio of GSH to its oxidized form, glutathione disulfide (GSSG), occurring under optimal growth conditions can be restored by means of higher glutathione reductase …
Link.springer.comThe Key Role of GSH in Keeping the Redox Balance in …
Nov 1, 2023 · Glutathione (GSH) is a ubiquitous tripeptide that is biosynthesized in situ at high concentrations (1–5 mM) and involved in the regulation of cellular homeostasis via multiple …
Ncbi.nlm.nih.govGlutathione Homeostasis and Functions: Potential Targets for …
Glutathione (GSH) is a tripeptide, which has many biological roles including protection against reactive oxygen and nitrogen species. The primary goal of this paper is to characterize the …
Onlinelibrary.wiley.comFacile and sensitive measurement of GSH/GSSG in cells by …
Mar 1, 2021 · A rapid, accurate measurement of cellular GSH and GSSG is quite needed in investigating important biochemical events. In this work, we present a novel and sensitive …
Sciencedirect.comGlutathione Metabolism and Its Function in Higher Plants
Mar 11, 2018 · Glutathione (GSH) is a low molecular weight tripeptide that plays an important role in metabolism and cell function. The cellular glutathione/glutathione disulfide (GSH/GSSG) …
Link.springer.comGlutathione reductase - Wikipedia
Glutathione reductase (EC 1.8.1.7) catalyzes the reduction of glutathione disulfide ( GSSG) to the sulfhydryl form glutathione ( GSH ), which is a critical molecule in resisting oxidative stress and …
En.wikipedia.orgGlutathione Metabolism and the Novel Role of Mitochondrial GSH …
Apr 24, 2021 · The oxidized form (GSSG) is predominantly produced by the catalysis of GSH peroxidase (GPx), as well as from the direct reactions of GSH with electrophilic compounds …
Ncbi.nlm.nih.govGSH/GSSG-Glo™ Assay | Measure Ratio of GSH to GSSG
The GSH/GSSG-Glo™ Assay is a luminescence-based system that detects and quantifies total glutathione (GSH + GSSG), GSSG and GSH-to-GSSG ratios in cultured cells. Reduced …
Promega.caDetermining glutathione and glutathione disulfide using the
Apr 10, 2000 · Because of the importance of glutathione (GSH) and glutathione disulfide (GSSG) in cellular signal transduction, gene regulation, redox regulation, and biochemical …
Pubmed.ncbi.nlm.nih.govThe dual role of FSP1 in programmed cell death: resisting …
3 days ago · The relative GSSG and GSH level in lysates was assessed by the T-GSH/GSSG assay kit (Nanjing Jiancheng Bioengineering Institute, A061-1-2). Reagent I, standards of GSH …
Molmed.biomedcentral.comRedox status expressed as GSH:GSSG ratio as a marker for …
Sep 21, 2012 · Reduced glutathione (GSH) is considered to be one of the most important scavengers of reactive oxygen species (ROS), and its ratio with oxidised glutathione (GSSG) …
Ncbi.nlm.nih.govFoliar-selenium enhances plant growth and arsenic
Foliar-Se promotes the GSH−GSSG cycle in P. vittata fronds. To better explore the mechanisms behind these observations, we determined the glutathione contents (GSH and GSSG), and …
Sciencedirect.comUpdate of the scientific opinion on tetrabromobisphenol A …
3 days ago · The first two opinions in the series updated the risk assessments of HBCDDs in food (EFSA CONTAM Panel, 2021) and PBDEs in food (EFSA CONTAM Panel, 2024).This third …
Efsa.onlinelibrary.wiley.com